The Spectral Variation Hypothesis (SVH) proposes that spectral heterogeneity (SH), derived from optical data, can serve as a proxy for estimating biodiversity. In this study, we tested the SVH across 42 forest plots in the Italian Alps using imaging spectroscopy data from the EnMAP satellite. We investigated the relationship between SH—quantified using two different metrics, Rao's Q and the coefficient of variation (CV)—and tree species diversity (using Shannon's H index and species richness). We applied three levels of spectral analysis: (1) SH calculated for each individual EnMAP band; (2) SH aggregated across broader spectral ranges (Visible -VIS-, Near Infrared -NIR-, and Shortwave Infrared -SWIR-) and (3) SH derived from vegetation indices (VIs). These analyses were performed under three spatial approaches: (A) a normal approach assigning equal weight to all four EnMAP pixels intersecting a plot; (B) a weighted approach based on the proportional overlap of each pixel with the plot area; and (C) a weighted canopy cover (CC)>70% approach, which included only plots with CC greater than 70% as derived from airborne laser scanning (ALS) LiDAR data. Weak to moderate correlations were observed when SH was derived from single bands, with the strongest relationships in the NIR (R² approaching 0.4), followed by the VIS and SWIR regions. A similar trend emerged when SH was aggregated across broader spectral ranges, with the highest correlations again found in the NIR (R² up to 0.35). In contrast, lower R² values were obtained when SH was computed from specific VIs. The weighted approaches, especially when restricted to plots with CC >70%, consistently yielded higher R² values than the equal-weight approach in all three the spectral analysis. Results were consistent across both SH metrics (Rao's Q and CV), with stronger correlations when species richness was used as the biodiversity metric. This work highlights how EnMAP hyperspectral data, despite inherent constraints, can provide valuable insights into forest biodiversity monitoring. © 2025 The Author(s)

Accurate estimates of aboveground biomass (AGB) are essential for forest policies to reduce carbon emissions. Unmanned aerial laser scanning (UAV-LS) offers unprecedented millimetric detail but is underutilized in monitoring broadleaf Mediterranean forests compared to coniferous ones. This study aims to design and evaluate a procedure for AGB estimates based on the predictive power of crown features. In the first step, we manually created Quantitative Structure Models (QSMs) for 320 trees using data from UAV laser scanning (UAV-LS), airborne laser scanning (ALS), and co-registered terrestrial laser scanning (TLS). This provided the most accurate non-destructive estimate of aboveground biomass (AGB) in the absence of destructive measurements. For each reference tree we also measured crown projection and crown volume to build two separated models relating AGB to such crown features. In the second phase, we evaluated the potential of UAV-LS for quantifying AGB in a pure European beech (Fagus sylvatica) forest and compared it with traditional ALS estimates, using fully automatic procedures. The two obtained tree-level AGB models were then tested using three datasets derived from 35 sampling plots over the same study area: (a) 1130 trees manually segmented (phase-2 reference); (b) trees automatically extracted from ALS data; and (c) trees automatically extracted from UAV-LS data. Results demonstrate that detailed UAV-LS data improve model sensitivity compared to ALS data (RMSE = 45.6 Mg ha⁻¹, RMSE% = 13.4%, R2 = 0.65, for the best ALS model; RMSE = 44.0 Mg ha⁻¹, RMSE% = 12.9%, R2 = 0.67, for the best UAV-LS model), allowing for the detection of AGB differences even in quite homogenous forest structures. Overall, this study demonstrates the combined use of both laser scanner data can foster non-destructive and more precise AGB estimation than the use of only one, in forested areas across hectare scales (1 to 100 ha). © 2025 by the authors.

The understory is an essential ecological and structural component of forest ecosystems. The lack of efficient, accurate, and objective methods for evaluating and quantifying the spatial spread of understory characteristics over large areas is a challenge for forest planning and management, with specific regard to biodiversity and habitat governance. In this study, we used terrestrial and airborne laser scanning (TLS and ALS) data to characterize understory in a European beech and black pine forest in Italy. First, we linked understory structural features derived from traditional field measurements with TLS metrics, then, we related such metrics to the ones derived from ALS. Results indicate that (i) the upper understory density (5–10 m above ground) is significantly associated with two ALS metrics, specifically the mean height of points belonging to the lower third of the ALS point cloud within the voxel (HM<inf>1/3</inf>) and the corresponding standard deviation (SD<inf>1/3</inf>), while (ii) for the lower understory layer (2–5 m above ground), the most related metric is HM<inf>1/3</inf> alone. As an example application, we have produced a map of forest understory for each layer, extending over the entire study region covered by ALS data, based on the developed spatial prediction models. With this study, we also demonstrated the power of hand-held mobile-TLS as a fast and high-resolution tool for measuring forest structural attributes and obtaining relevant ecological data. © 2023 by the authors.