The understory is an essential ecological and structural component of forest ecosystems. The lack of efficient, accurate, and objective methods for evaluating and quantifying the spatial spread of understory characteristics over large areas is a challenge for forest planning and management, with specific regard to biodiversity and habitat governance. In this study, we used terrestrial and airborne laser scanning (TLS and ALS) data to characterize understory in a European beech and black pine forest in Italy. First, we linked understory structural features derived from traditional field measurements with TLS metrics, then, we related such metrics to the ones derived from ALS. Results indicate that (i) the upper understory density (5–10 m above ground) is significantly associated with two ALS metrics, specifically the mean height of points belonging to the lower third of the ALS point cloud within the voxel (HM<inf>1/3</inf>) and the corresponding standard deviation (SD<inf>1/3</inf>), while (ii) for the lower understory layer (2–5 m above ground), the most related metric is HM<inf>1/3</inf> alone. As an example application, we have produced a map of forest understory for each layer, extending over the entire study region covered by ALS data, based on the developed spatial prediction models. With this study, we also demonstrated the power of hand-held mobile-TLS as a fast and high-resolution tool for measuring forest structural attributes and obtaining relevant ecological data. © 2023 by the authors.

Vegetation structure is one of the key factors in forest ecosystems. Especially understory structure has major implications for wildlife habitat selection, reproduction, and survival. Structural indices traditionally used to characterize understory vegetation are based on field vegetation surveys describing general features such as leaf area index (LAI), canopy cover or vegetation height, hiding much of the three-dimensional vegetation structure complexity. The application of terrestrial laser scanning (TLS) in forest ecological and management applications is becoming more effective. In this study, we use TLS data to quantify spatial attributes of forest subcanopy in four different forest strata ranging from 0.5 m to 10 m from the ground. We collected data in 12 plots of mature European beech (Fagus sylvatica L.) forests and 12 plots of mature black pine (Pinus nigra subsp. laricio Maire) forests, located in the Sila National Park, Italy. We propose a TLS-based approach to estimate a fine-scale vegetation density using the Plant Density Index (PDI) and to test the PDI at different height classes. We found a significant relationship between the PDI and the number of trees belonging to the dominant layer, using the Spearman correlation coefficient (r = 0.83, p<inf>val</inf> = 0.001). Basing on PDI values, a cluster analysis of the four subcanopy strata was carried out for deriving clusters of structurally homogeneous forest plots. Results identified three clusters in terms of the vegetation features in the horizontal height classes: the first cluster primarily includes Beech forests characterized by plots with the highest tree densities; the second one includes both Beech and Pine forests characterized by dense ground vegetation and shrubs and an intermediate tree density; the third group is represented by Pine forests with massive presence of vegetation lower strata and moderate tree density. Then, PCA allowed identifying the relationship between the considered subcanopy layers and forest plots. © 2021 Elsevier B.V.