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Pubblicazioni Scientifiche
Filtri di ricerca 16 risultati
Pubblicazioni per anno
Estimating canopy and stand structure in hybrid poplar plantations from multispectral UAV imagery
Romano
,
Elio
,
Brambilla
,
Massimo
,
Chianucci
,
Francesco
,
Tattoni
,
Clara
,
Puletti
,
Nicola
,
Chirici
,
Gherardo
,
Travaglini
,
Davide
,
Giannetti
,
Francesca
canopy photography
canopy structure
poplar plantation
texture metrics
digital camera
gclm metrics
unmanned aerial vehicle
Mostra abstract
Accurate estimates of canopy structure like canopy cover (CC), Leaf Area Index (LAI), crown volume (Vcr), as well as tree and stand structure like stem volume (V_st) and basal area (G), are considered essential measures to manage poplar plantations effectively as they are correlated with the growth rate and the detection of possible stress. This research exploits the possibility of developing a precision forestry application using an unmanned aerial vehicle (UAV), terrestrial digital camera and traditional field measurements to monitor poplar plantation variables. We set up the procedure using explanatory variables from the Grey Level Co-occurrence Matrix textural metrics (Entropy, Variance, Dissimilarity and Contrast) calculated based on UAV multispectral imagery. Our results show that the GCLM texture derived by multispectral ortomosaic provides adequate explanatory variables to predict poplar plantation characteristics related to plants' canopy and stand structure. The evaluation of the models targeting the different poplar plantation variables (i.e. Vcr, G_ha, Vst_ha, CC and LAI) with the four GLCM explanatory variables (i.e. Entropy, Variance, Dissimilarity and Contrast) consistently higher or equal resulted to R<sup>2</sup> ≥0.86. © 2024, Editura Silvica. All rights reserved.
Estimated biomass loss caused by the vaia windthrow in northern italy: Evaluation of active and passive remote sensing options
Vaglio Laurin
,
Gaia
,
Puletti
,
Nicola
,
Tattoni
,
Clara
,
Ferrara
,
Carlotta
,
Pirotti
,
Francesco
Mostra abstract
Windstorms are a major disturbance factor for European forests. The 2018 Vaia storm, felled large volumes of timber in Italy causing serious ecological and financial losses. Remote sensing is fundamental for primary assessment of damages and post‐emergency phase. An explicit estimation of the timber loss caused by Vaia using satellite remote sensing was not yet undertaken. In this investigation, three different estimates of timber loss were compared in two study sites in the Alpine area: pre‐existing local growing stock volume maps based on lidar data, a recent national‐level forest volume map, and an novel estimation of AGB values based on active and passive remote sensing. The compared datasets resemble the type of information that a forest manager might potentially find or produce. The results show a significant disagreement in the different biomass estimates, related to the methods used to produce them, the study areas characteristics, and the size of the damaged areas. These sources of uncertainty highlight the difficulty of estimating timber loss, unless a unified national or regional European strategy to improve preparedness to forest hazards is defined. Considering the frequent impacts on forest resources that occurred in the last years in the European Union, remote sensing‐based surveys targeting forests is urgent, particularly for the many European countries that still lack reliable forest stocks data. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Influence of image pixel resolution on canopy cover estimation in poplar plantations from field, aerial and satellite optical imagery
Chianucci
,
Francesco
,
Puletti
,
Nicola
,
Grotti
,
Mirko
,
Bisaglia
,
Carlo
,
Giannetti
,
Francesca
,
Romano
,
Elio
,
Brambilla
,
Massimo
,
Mattioli
,
Walter
,
Cabassi
,
Giovanni
,
Bajocco
,
Sofia
,
Li
,
Linyuan
,
Chirici
,
Gherardo
,
Corona
,
P.
,
Tattoni
,
Clara
Mostra abstract
Accurate estimates of canopy cover (CC) are central for a wide range of forestry studies. As direct measurements are impractical, indirect optical methods have often been used to estimate CC from the complement of gap fraction measurements obtained with restricted-view sensors. In this short note we evaluated the influence of the image pixel resolution (ground sampling distance; GSD) on CC estimation in poplar plantations obtained from field (cover photography; GSD < 1 cm), unmanned aerial (UAV; GSD <10 cm) and satellite (Sentinel-2; GSD = 10 m) imagery. The trial was conducted in poplar tree plantations in Northern Italy, with varying age and canopy cover. Results indicated that the coarser resolution available from satellite data is suitable to obtain estimates of canopy cover, as compared with field measurements obtained from cover photography; therefore, S2 is recommended for larger scale monitoring and routine assessment of canopy cover in poplar plantations. The higher resolution of UAV compared with Sentinel-2 allows finer assessment of canopy structure, which could also be used for calibrating metrics obtained from coarser-scale remote sensing products, avoiding the need of ground measurements. © 2021 Centro di Ricerca per la Selvicoltura, Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria. All rights reserved.
Nondestructive tree stem and crown volume allometry in hybrid poplar plantations derived from terrestrial laser scanning
Chianucci
,
Francesco
,
Puletti
,
Nicola
,
Grotti
,
Mirko
,
Ferrara
,
Carlotta
,
Giorcelli
,
Achille
,
Coaloa
,
Domenico
,
Tattoni
,
Clara
Mostra abstract
Accurate and frequently updated tree volume estimates are required for poplar plantations, which are characterized by fast growth rate and short rotation. In this study, we tested the potential of terrestrial laser scanning (TLS) as a reliable method for developing nondestructive tree volume allometries in poplar plantations. The trial was conducted in Italy, where 4- to 10-year-old hybrid plantations were sampled to develop tree crown volume allometry in leaf-on conditions, tree stem volume, and height-diameter allometries in leaf-off conditions. We tested one-entry models based on diameter and two-entry models based on both diameter and height. Model performance was assessed by residual analysis. Results indicate that TLS can provide accurate models of tree stem and crown volume, with percentage of root-mean-square error of about 20 percent and 15 percent, respectively. The inclusion of height does not bring relevant improvement in the models, so that only diameter can be used to predict tree stem and crown volume. The TLS-measured stem volume estimates agreed with an available formula derived from harvesting. We concluded that TLS is a reliable method for developing nondestructive volume allometries in poplar plantations and holds great potential to enhance conventional tree inventory and monitoring. © The Author(s) 2020. Published by Oxford University Press on behalf of the Society of American Foresters. All rights reserved.
A PLOT SAMPLING STRATEGY FOR ESTIMATING THE AREA OF OLIVE TREE CROPS AND OLIVE TREE ABUNDANCE IN A MEDITERRANEAN ENVIRONMENT
Grotti
,
Mirko
,
Puletti
,
Nicola
,
Chianucci
,
Francesco
,
Mattioli
,
Walter
,
Floris
,
Antonio
,
Clementel
,
Fabrizio
,
Torresan
,
C.
,
Marchi
,
Maurizio
,
Gentile
,
Alessandra
,
Pisante
,
Michele
,
Marcelli
,
Agnese
,
Corona
,
P.
Mostra abstract
Accurate inventory and mapping of olive (Olea europaea L.) tree attributes represents a central issue to support the olive production system. With reference to the cultivation, there is a high heterogeneity and complexity in the cultivation of olive trees, which is reflected in the large variability in olive grove surfaces. This poses some challenge in accurately estimating olive tree attributes via traditional inventory approaches, as commonly adopted in national forest inventory. From a methodological point of view, the complexity and heterogeneity of olive tree groves can be comparable to the problem of accurately estimating tree outside forests (TOF) attributes. In this study, we tested whether a plot sampling approach formerly developed for TOF is suitable for estimating olive tree attributes at large scale. We tested this approach in a case study where the census of the olive crop area and the number of olive groves was conducted from photo-interpretation of high resolution aerial orthoimagery, used as benchmark to test the effectiveness of the plot sampling approach. The main result of this study is that the plot sampling method can be applied for estimating olive tree attributes. Our obtained RSEs were below 20%, with a limited sampling effort of about 6% of the studied population; the obtained RSEs were below 6% when increasing sampling up to about 21% the studied population. Using robust statistical procedures among countries, should allow obtaining harmonized and comparable information, which can increase the knowledge of olive geographical distribution and structure at its relevant Mediterranean scale. © 2019, Italian Society of Remote Sensing. All rights reserved.
Indicators for the assessment and certification of cork oak management sustainability in Italy
Pollastrini
,
M.
,
Chiavetta
,
U.
,
Cutini
,
Andrea
,
Casula
,
Antonio
,
Maltoni
,
Sara
,
Dettori
,
Sandro
,
Corona
,
P.
italy
forest management planning
non-wood forest products
quercus suber
sardinia
sustainable forest management
Mostra abstract
Sustainable forest management (SFM) is crucial for forest ecosystem productivity and conservation, especially in systems such as cork oak (Quercus suber L.) threatened by human activities and biotic and abiotic factors. In this study SFM indicators with particular reference to cork oak forests in the region of Sardinia (Italy) are proposed and tested. Sustainable and responsible management options specifically aimed at cork oak forest management and chain of custody certification are also provided. A set of ten indicators was proposed and assessed by an expert panel in cork oak management. Five indicators were also tested against data on structure, origin, health condition and management in 285 forest compartments managed by FoReSTAS (Regional Forest Agency for Land and Environment of Sardinia, Italy), including 361 sampling plots and 5345 trees. In order to investigate the priorities and perceptions of SFM experts and stakeholders, a survey was also carried out by completion of a questionnaire on the technical issues of cork oak woodland management. The survey results highlighted a need to improve environmental and economic performance by means of SFM and certification. The indicators tested in Sardinian cork oak woodlands showed that about 80% of the stands fulfilled management sustainability requirements. The suggested SFM indicators can effectively support proactive management and conservation measures, representing a valuable tool in the current context of growing environmental and socioeconomic awareness. © SISEF.
Behaviour of Brown Bears Under Fluctuating Resource Availability
Tattoni
,
Clara
,
Corradini
,
Andrea
,
Chianucci
,
Francesco
,
Ciolli
,
Marco
,
Giusti
,
Roberta
,
Bragalanti
,
Natalia
,
Cagnacci
,
Francesca
,
Martinoli
,
Adriano
,
Preatoni
,
Damiano G.
,
Bisi
,
Francesco
Mostra abstract
Mast seeding, the variable and intermittent production of seeds, has cascading effects on ecosystem functioning. This study explores its influence on the brown bear populations in the Italian Alps, focusing on beechnuts (Fagus sylvatica L.), the primary food source for bears in the region. Using historical data and field sampling, we estimated and mapped the annual seed biomass from 2007 to 2021 for the province of Trento. The energy content of beechnuts was assessed through high heating values, providing the caloric resources available. Data on beechnuts production, records of damages and GPS data from 16 Eurasian brown bears were integrated to perform a temporal and spatial analysis at home range and at landscape level. Standardised damages to beehives and livestock decreased during mast years, suggesting that bears met their trophic needs through natural food sources. In fact, bears used more agricultural areas and less beech forest during years of beech crop failure. At landscape level, agriculture and pasture areas close to beech forests and distant from cities showed a higher risk of damage, providing a tool to anticipate management actions. This work provides insights on the ecological dynamics and conservation implications of brown bears in the study area by mapping the spatial and temporal aspects of mast seeding and bear-related damages. © 2025 The Author(s). Ecology and Evolution published by British Ecological Society and John Wiley & Sons Ltd.
Repeated mapped tree inventory in an oak-hornbeam planted forest in Po Valley (Foresta Carpaneta, Italy)
Mostra abstract
Stand structure and tree spatial patterns are key elements to understand natural dynamics and competition processes in forest ecosystems. We performed repeated, mapped tree inventory measures (x, y, height, diameter, vitality, etc.) to allow analysis of the spatial and temporal structure and diversity in 1 ha oak-hornbeam planted forest with pedunculate oak (Quercus robur L.), established in 2003 also for the conservation of a variety of oak genotypes. Two inventories were carried out in 2009 and 2019. The use of repeated and mapped tree measures allows to investigate the changes in spatial pattern processes through time in this forest. © 2024 Istituto Sperimentale per la Selvicoltura. All rights reserved.
Evaluating sampling schemes for quantifying seed production in beech (Fagus sylvatica) forests using ground quadrats
Chianucci
,
Francesco
,
Tattoni
,
Clara
,
Ferrara
,
Carlotta
,
Ciolli
,
Marco
,
Brogi
,
Rudy
,
Zanni
,
Michele
,
Apollonio
,
Marco
,
Cutini
,
Andrea
Mostra abstract
Accurate estimates of seed production are central for understanding mast seeding mechanisms at tree and forest scales, and for designing sustainable management strategies. As trees are long-lived organisms, a long-term perspective is required to understand how reproduction acts during the life cycle of a tree. However, long-term series of seed production are challenging to obtain, as the available seed count procedures strictly rely on field methods, which are cost- and time-consuming, inherently limiting their widespread use at extensive spatial and temporal scales. In this study, we proposed a simple, rapid and flexible field method based on counting the seed in mobile ground quadrats (GQ), which was tested in beech forests. Quadrat measurements were first validated against reference measurements obtained from litter traps (LT) in three permanent plots. Results indicated that GQ provides robust and reliable estimates of seeds, which are not affected by seed predation occurring at the forest floor. Additional quadrat measurements were performed to evaluate the influence of sampling schemes (random, regular, systematic) on the estimation of mean seed production at the plot scale. One hundred quadrats were collected in 0.25 ha beech plots and considered as a reference for evaluating the different sampling schemes and sampling sizes. Measurements were performed in October (three plots), which represented the peak of seed fall, and November (two plots). Results indicate that about 25 randomly located measurements allowed to characterize plot-level mean seed production with an acceptable error below 20%, regardless of the different mean seed production observed between the studied plots and the sampling periods. If the 25 sampling points are arranged in a grid, the obtained mean estimates are within the confidence interval of the reference plot-level values. © 2021 Elsevier B.V.
A comparison of ground-based count methods for quantifying seed production in temperate broadleaved tree species
Tattoni
,
Clara
,
Chianucci
,
Francesco
,
Ciolli
,
Marco
,
Ferrara
,
Carlotta
,
Marchino
,
Luca
,
Zanni
,
Michele
,
Zatelli
,
Paolo
,
Cutini
,
Andrea
Mostra abstract
• Key message: Litter trap is considered the most effective method to quantify seed production, but it is expensive and time-consuming. Counting fallen seeds using a quadrat placed on the ground yields comparable estimates to the litter traps. Ground quadrat estimates derived from either visual counting in the field or image counting from quadrat photographs are comparable, with the latter being also robust in terms of user sensitivity. • Context: Accurate estimates of forest seed production are central for a wide range of ecological studies. As reference methods such as litter traps (LT) are cost- and time-consuming, there is a need of fast, reliable, and low-cost tools to quantify this variable in the field. • Aims: To test two indirect methods, which consist of counting the seeds fallen in quadrats. • Methods: The trial was performed in three broadleaved (beech, chestnut, and Turkey oak) tree species. Seeds are either manually counted in quadrats placed at the ground (GQ) or from images acquired in the same quadrats (IQ) and then compared against LT measurements. • Results: GQ and IQ provide fast and reliable estimates of seeds in both oak and chestnut. In particular, IQ is robust in terms of user sensitivity and potentially enables automation in the process of seed monitoring. A null-mast year in beech hindered validation of quadrats in beech. • Conclusion: Quadrat counting is a powerful tool to estimate forest seed production. We recommend using quadrats and LT to cross-calibrate the two methods in case of estimating seed biomass. Quadrats could then be used more routinely on account of their faster and simpler procedure to obtain measurements at more spatially extensive scales. © 2021, The Author(s).
Dataset of tree inventory and canopy structure in poplar plantations in Northern Italy
Chianucci
,
Francesco
,
Marchino
,
Luca
,
Bidini
,
Claudio
,
Giorcelli
,
Achille
,
Coaloa
,
Domenico
,
Chiarabaglio
,
Pier Mario
,
Giannetti
,
Francesca
,
Chirici
,
Gherardo
,
Tattoni
,
Clara
Mostra abstract
The dataset reports data collected in 38 square (50 x 50m) 0.25 ha plots representative of poplar plantations in Lombardy Region (Northern Italy), which were used to calibrate optical information derived from unmanned aerial vehicle (UAV) and satellite (Sentinel-2) sensors. In each plot, the diameter at breast height was measured using a caliper; height, stem and crown volume of each tree were then derived from diameter using allometric equations developed in an independent study. Additional canopy attributes (foliage and crown cover, crown porosity, leaf area index) were derived in each plot from 12-20 optical images collected using digital cover photography (DCP). The collected data allows characterizing the assessment of structure of these plantations, along with their variation over the rotation time. Canopy and crown data also enable the evaluation of optimal rotation and tree spacing, as well as the relationship between stand and canopy structure. The raw datasets consist of 2,591 records (trees) associated with inventory measurements and 616 records (images) associated with optical canopy measurements. An R code was also provided to calculate plot-level attributes from raw data. Dataset and associated metadata are freely available at http://dx.doi.org/10.17632/ycr7w5pvkt.1. © 2021 Centro di Ricerca per la Selvicoltura, Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria. All rights reserved.
Multi-temporal dataset of stand and canopy structural data in temperate and Mediterranean coppice forests
Chianucci
,
Francesco
,
Ferrara
,
Carlotta
,
Bertini
,
Giada
,
Fabbio
,
Gianfranco
,
Tattoni
,
Clara
,
Rocchini
,
Duccio
,
Corona
,
P.
,
Cutini
,
Andrea
Mostra abstract
Key message: We provided long-term stand and canopy structural data from permanent monitoring plots representative of some most diffuse temperate and Mediterranean forests, under different coppice management regimes. Periodic inventories were performed in the surveyed plots since the 1970s. Annual litterfall production and its partitioning (leaf, woody, reproductive parts) and optical canopy measurements using the LAI-2000 Plant Canopy Analyzer were performed every year in fully equipped plots since the 1990s. These data can be used for evaluating the influence of coppice management in the stand and canopy structure, the parametrization of radiative transfer models that require accurate ground truth data, and the calibration of high to medium resolution remotely sensed data. Dataset access is at https://doi.org/10.17632/z8zm3ytkcx.2. Associated metadata is available at https://agroenvgeo.data.inra.fr/geonetwork/srv/eng/catalog.search#/metadata/2bd2d77f-3cf8-43da-b1b5-9f8196dc017f . © 2019, INRA and Springer-Verlag France SAS, part of Springer Nature.
LONG-TERM COMPARISON OF IN SITU AND REMOTELY-SENSED LEAF AREA INDEX IN TEMPERATE AND MEDITERRANEAN BROADLEAVED FORESTS
Tattoni
,
Clara
,
Chianucci
,
Francesco
,
Grotti
,
Mirko
,
Zorer
,
Roberto
,
Cutini
,
Andrea
,
Rocchini
,
Duccio
Mostra abstract
Monitoring vegetation structure and functioning is critical for modelling terrestrial ecosystems and energy cycles. Leaf area index (LAI) is an important structural property of vegetation used in many land-surface, climate, and forest monitoring applications. Remote sensing provides a unique way to obtain estimates of leaf area index at spatially extensive areas. However, the analysis and extraction of quantitative information from remotely-sensed data require accurate cross-calibration with in situ forest measurements, which are generally spatially-and temporally-limited, thereby limiting the ability to compare the seasonal dynamic patterns between field and remotely-sensed time series. This is particularly relevant in temperate broadleaved forests, which are characterized by high level of complexity, which can complicate the retrieval of vegetation attributes from remotely-sensed data. In this study, we performed a long-term comparison of MODIS LAI products with continuous in situ leaf area index measurements collected monthly in temperate and Mediterranean forests from 2000 to 2016. Results indicated that LAI showed a good correlation between satellite and ground data for most of the stands, and the pattern in seasonal changes were highly overlapping between the time-series. We conclude that MODIS LAI data are suitable for phenological application and for up-scaling LAI from the stand level to larger scales. © 2019, Italian Society of Remote Sensing. All rights reserved.
Reproductive phenology and conception synchrony in a natural wild boar population
Canu
,
Antonio
,
Scandura
,
Massimo
,
Merli
,
Enrico
,
Chirichella
,
Roberta
,
Bottero
,
Elisa
,
Chianucci
,
Francesco
,
Cutini
,
Andrea
,
Apollonio
,
Marco
Mostra abstract
Reproductive synchrony among gregarious mammals has a strong adaptive value and may lead to cooperative behaviors aimed at maximizing offspring survival. Additionally, temporal clustering of estrus has important implications on individual mating tactics and ultimately affects the degree of polygamy in a population. Although several studies have examined the reproductive biology of wild boar (Sus scrofa), much remains to be understood about the patterns of timing and synchrony of reproduction in natural populations. We analyzed the spatiotemporal distribution of conception dates in an Italian wild boar population taking into account the effects of environmental and individual factors, in order to determine the main variables influencing the timing of reproduction and to detect the signs of a socially-driven reproductive synchrony. Specifically, for each litter belonging to 354 pregnant sows culled between 2006 and 2013 in a mountain area of Tuscany, we determined the conception date (CD) from an estimate of the mean fetal age and the culling date. We then investigated which factors drove the variation in CD, by implementing linear mixed models, Mantel tests and spatial autocorrelation analyses. The selected model showed significant effects of rainfall, temperatures, and previous and current productivity on CD, as well as a strong correlation of CDs among sows culled in close spatial and temporal proximity (i.e., in the same hunting ground and hunting season). Likewise, autocorrelation analyses and Mantel tests consistently indicated that closer sows had similar conception dates. Overall, our results confirm the effect of resource availability and climate on wild boar reproductive phenology, and suggest socially-driven reproductive patterns, in spite of a high turn-over in social groups due to hunting. Finally, possible advantages and evolutionary implications of reproductive synchrony in wild boar are discussed. © 2015 Associazione Teriologica Italiana.
Stand structure and ecological changes in holm oak coppices 25 years later the opening of thinning operations for the conversion into high forest; Cambiamenti strutturali ed ecologici in cedui di leccio in Sardegna a 25 anni dal taglio di avviamento ad altofusto
Manetti
,
Maria Chiara
,
Giannini
,
Tessa
,
Chianucci
,
Francesco
,
Casula
,
Antonio
,
Cutini
,
Andrea
Mostra abstract
Holm oak (Quercus ilex L.) is one of the most diffuse and economically important forest species in Sardinia, where it holds about 40% of holm oak cover in Italy. The forest type has also acquired a high ecological, recreational and landscape value over the last decades. Most of holm oak stands originated from overgrown coppice forests partly undergoing conversion into high forest. This study was set up in 1994 to analyse, as a function of site-index, the effects of conversion thinning on productivity, biodiversity, structural dynamics and canopy characteristics in an holm oak forest located in southern Sardinia. Two experimental permanent plots, differing in site index, stand structure and tree density, were established. The surveys were carried out in 1994-95 and 2010-11. The analysis included growth pattern, dynamics of stand structure and estimation of forest canopy attributes as leaf area index and canopy transmittance. Results pointed out the simplified stand structure, the poor biodiversity, the low LAI and high transmittance values 9 years after thinning implementation. These characteristics were more pronounced in the less productive area, characterised by substantial canopy gaps. 25 years after thinning implementation, both stands showed significant increase in the number of trees, strengthening of the clustered structure and high canopy recovery. Conversely, no significant changes in biodiversity and vertical structure were observed. Overall results contributed to a positive evaluation of the conversion practice based on periodical thinnings, even if the excessive reduction of tree density, mainly in the lower site-index area, did not allow yet the fully achievement of canopy recovery.
Ecological portrayal of old-growth forests and persistent woodlands in the cilento and vallo di Diano National Park (southern Italy)
Marchetti
,
Marco
,
Tognetti
,
Roberto
,
Lombardi
,
Fabio
,
Chiavetta
,
U.
,
Palumbo
,
Giuseppe
,
Sellitto
,
Vincenzo Michele
,
Colombo
,
C. Massimo
,
Iovieno
,
Paola
,
Alfani
,
Anna
,
Baldantoni
,
Daniela
,
Barbati
,
Anna
,
Ferrari
,
Barbara
,
Bonacquisti
,
Sandro
,
Capotorti
,
Giulia
,
Copiz
,
Riccardo
,
Blasi
,
Carlo
biodiversity
mediterranean region
ecological characterization
managed stands
old-growth forests
persistent woodlands
soil
Mostra abstract
The maintenance of certain levels of old forest represents a cornerstone of the EU's biodiversity management strategy. A consensus on a single general ecological definition of old-growth is particularly difficult in Mediterranean Europe. The present paper deals with old-growth forests and persistent woodlands in the Cilento and Vallo di Diano National Park (PNCVD) to give an ecological understanding of forest complexity and dynamics under a multiscale and multidisciplinary perspective. The multiscale approach ranged from the identification and mapping of potential old-growth stands at landscape scale to a two-level field review of forest stand features. Field sampling involved a multidisciplinary team of researchers in forest structure, pedologic environment, soil microbial activity, flora and vegetation and deadwood components. The research provided sound knowledge about old-growthness features in the PNCVD that constitutes a unique case study in the whole Mediterranean basin. The integration of results allowed to: identify main ecosystem functions and the related services of the old-growth forests in the study area; distinguish persistent woodlands, multi-aged stands with old trees deriving from nineteenth-century management practices, from old-growth forests sensu strictu; recognize indicators of direct and indirect impacts of human activities; suggest effective practices for sustainable management in the Mediterranean context. © 2010 Società Botanica Italiana.